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human stem cell factor  (R&D Systems)


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    R&D Systems human stem cell factor
    Human Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+stem+cell+factor+scf/Recombinant+Human+SCF+Protein%2C+CF/pm41963354-280-62-67
    Average 94 stars, based on 8 article reviews
    human stem cell factor - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Modification:

    Article Title: Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes49. .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Article Title: Functional genomics using CRISPR-Cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes (49). .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL halo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Article Title: Method for producing highly functional platelets
    Article Snippet: .. At this time, medium obtained by further adding protamine to a final concentration of 10 μg/mL to medium containing 50 ng/mL of human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL of human stem cell factor (SCF) (R&D Systems, Inc.) and 2 μg/mL of doxycycline (Dox) in a basal medium (IMDM (Iscove's modified Dulbecco's medium) (Sigma-Aldrich Company Ltd.) containing 15% fetal bovine serum (Gibco Corporation), 1% penicillin-streptomycin-glutamine (Gibco Corporation), 1% insulin, transferrin and selenium solution (ITS-G) (Gibco Corporation), 0.45 mM 1-thioglycerol (Sigma-Aldrich Company Ltd.) and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Company Ltd.)) (to be referred to as differentiation medium) was used for the medium. .. Furthermore, the lentivirus vector was a tetracycline-regulated inducible vector produced by recombining the mOKS cassette of LV-TRE-mOKS-Ubc-tTA-I2G (Kobayashi, T., et al., Cell, 142, 787-799 (2010)) in c-MYC, BMI1 and BCL-xL (respectively referred to as LV-TRE-c-Myc-Ubc-tTA-I2G, LV-TRE-BMI1-Ubc-tTA-I2G and LV-TRE-BCL-xL-Ubc-tTA-I2G).

    Article Title: Method for producing culture containing megakaryocytes, and method for producing platelets using same
    Article Snippet: .. The medium used here was obtained by the addition of protamine at a final concentration of 10 μg/mL to a medium (referred to below as the differentiation medium) prepared by the incorporation of 50 ng/mL human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL human stem cell factor (SCF) (R&D Systems, Inc.), and 2 μg/mL doxycycline (Dox) into a base medium (IMDM (Iscove's Modified Dulbecco's Medium) (Sigma-Aldrich Corporation) containing 15% fetal bovine serum (GIBCO), 1% penicillin-streptomycin-glutamine (GIBCO), 1% insulin, transferrin, selenium solution (ITS-G) (GIBCO), 0.45 mM 1-thioglycerol (Sigma-Aldrich Corporation), and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Corporation)). ..

    Article Title: Method for producing culture containing megakaryocytes, and method for producing platelets using same
    Article Snippet: .. After infection, the immortalized megakaryocyte cell line was static cultured under conditions of 37° C. and 5% CO2 using a culture medium (puromycin-containing differentiation medium) provided by the incorporation of 2 μg/mL puromycin into a medium (differentiation medium) that contained 50 ng/mL human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL human stem cell factor (SCF) (R&D Systems, Inc.), and 2 μg/mL doxycycline (Dox) in a base medium (IMDM (Iscove's Modified Dulbecco's Medium) (Sigma-Aldrich Corporation) containing 15% fetal bovine serum (GIBCO), 1% penicillin-streptomycin-glutamine (GIBCO), 1% insulin, transferrin, selenium solution (ITS-G) (GIBCO), 0.45 mM 1-thioglycerol (Sigma-Aldrich Corporation), and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Corporation)). ..

    Recombinant:

    Article Title: Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes49. .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Article Title: Functional genomics using CRISPR-Cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes (49). .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL halo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Solvent:

    Article Title: Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes49. .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Article Title: Functional genomics using CRISPR-Cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes (49). .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL halo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Clinical Proteomics:

    Article Title: Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes49. .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Article Title: Functional genomics using CRISPR-Cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
    Article Snippet: 1 μg/mL doxycycline (Sigma) was included in the culture to induce expression of the human papilloma virus type 16 E6/E7 genes (49). .. HUDEP-2 cells were differentiated in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 330 μg/mL halo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma), 5% human solvent detergent pooled plasma AB (Rhode Island Blood Center), 3 IU/mL erythropoietin (Amgen), 100 ng/mL human stem cell factor (SCF) (R&D), 1 μg/mL doxycycline (Sigma), 1% L-glutamine (Life Technologies), and 2% penicillin/streptomycin (Life Technologies). ..

    Cell Culture:

    Article Title: Regulation of platelet numbers and sizes by signaling pathways.
    Article Snippet: .. Either the glycoprotein (GP) Ib deficiency or hyper-function in humans can cause macrothrombocytopenia, the molecular mechanisms of which remain unclear.. Herein, the investigations for disease pathogenesis were performed in the human induced pluripotent stem cell (hiPSC) model.. The hiPSCs carrying a gain-of-function GP1BA p.M255V mutation which was described in platelet-type von Willebrand disease (PT-VWD) were generated using CRISPR/Cas9. ..

    Article Title: Method for producing culture containing megakaryocytes, and method for producing platelets using same
    Article Snippet: .. After infection, the immortalized megakaryocyte cell line was static cultured under conditions of 37° C. and 5% CO2 using a culture medium (puromycin-containing differentiation medium) provided by the incorporation of 2 μg/mL puromycin into a medium (differentiation medium) that contained 50 ng/mL human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL human stem cell factor (SCF) (R&D Systems, Inc.), and 2 μg/mL doxycycline (Dox) in a base medium (IMDM (Iscove's Modified Dulbecco's Medium) (Sigma-Aldrich Corporation) containing 15% fetal bovine serum (GIBCO), 1% penicillin-streptomycin-glutamine (GIBCO), 1% insulin, transferrin, selenium solution (ITS-G) (GIBCO), 0.45 mM 1-thioglycerol (Sigma-Aldrich Corporation), and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Corporation)). ..

    Concentration Assay:

    Article Title: Method for producing highly functional platelets
    Article Snippet: .. At this time, medium obtained by further adding protamine to a final concentration of 10 μg/mL to medium containing 50 ng/mL of human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL of human stem cell factor (SCF) (R&D Systems, Inc.) and 2 μg/mL of doxycycline (Dox) in a basal medium (IMDM (Iscove's modified Dulbecco's medium) (Sigma-Aldrich Company Ltd.) containing 15% fetal bovine serum (Gibco Corporation), 1% penicillin-streptomycin-glutamine (Gibco Corporation), 1% insulin, transferrin and selenium solution (ITS-G) (Gibco Corporation), 0.45 mM 1-thioglycerol (Sigma-Aldrich Company Ltd.) and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Company Ltd.)) (to be referred to as differentiation medium) was used for the medium. .. Furthermore, the lentivirus vector was a tetracycline-regulated inducible vector produced by recombining the mOKS cassette of LV-TRE-mOKS-Ubc-tTA-I2G (Kobayashi, T., et al., Cell, 142, 787-799 (2010)) in c-MYC, BMI1 and BCL-xL (respectively referred to as LV-TRE-c-Myc-Ubc-tTA-I2G, LV-TRE-BMI1-Ubc-tTA-I2G and LV-TRE-BCL-xL-Ubc-tTA-I2G).

    Article Title: Method for producing culture containing megakaryocytes, and method for producing platelets using same
    Article Snippet: .. The medium used here was obtained by the addition of protamine at a final concentration of 10 μg/mL to a medium (referred to below as the differentiation medium) prepared by the incorporation of 50 ng/mL human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL human stem cell factor (SCF) (R&D Systems, Inc.), and 2 μg/mL doxycycline (Dox) into a base medium (IMDM (Iscove's Modified Dulbecco's Medium) (Sigma-Aldrich Corporation) containing 15% fetal bovine serum (GIBCO), 1% penicillin-streptomycin-glutamine (GIBCO), 1% insulin, transferrin, selenium solution (ITS-G) (GIBCO), 0.45 mM 1-thioglycerol (Sigma-Aldrich Corporation), and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Corporation)). ..

    Infection:

    Article Title: Method for producing culture containing megakaryocytes, and method for producing platelets using same
    Article Snippet: .. After infection, the immortalized megakaryocyte cell line was static cultured under conditions of 37° C. and 5% CO2 using a culture medium (puromycin-containing differentiation medium) provided by the incorporation of 2 μg/mL puromycin into a medium (differentiation medium) that contained 50 ng/mL human thrombopoietin (TPO) (R&D Systems, Inc.), 50 ng/mL human stem cell factor (SCF) (R&D Systems, Inc.), and 2 μg/mL doxycycline (Dox) in a base medium (IMDM (Iscove's Modified Dulbecco's Medium) (Sigma-Aldrich Corporation) containing 15% fetal bovine serum (GIBCO), 1% penicillin-streptomycin-glutamine (GIBCO), 1% insulin, transferrin, selenium solution (ITS-G) (GIBCO), 0.45 mM 1-thioglycerol (Sigma-Aldrich Corporation), and 50 μg/mL L-ascorbic acid (Sigma-Aldrich Corporation)). ..



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